3TID

Crystal structure of the LCMV derived peptide GP34 in complex with the murine mhc class I H-2 Kb

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	200
Detector technology	CCD
Collection date	2010-10-20
Detector	ADSC QUANTUM 315
Wavelength(s)	1.089
Spacegroup name	C 1 2 1
Unit cell lengths	172.261, 47.649, 70.539
Unit cell angles	90.00, 106.39, 90.00

Refinement procedure

Resolution	39.376 - 1.650
R-factor	0.1762
Rwork	0.175
R-free	0.19220
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.164
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.000	45.000	1.710
High resolution limit [Å]	1.650	3.550	1.650
Number of reflections	64779
Completeness [%]	97.0	98.3	95.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	PEG 8K, Cacodylate, Magnesium Acetate, DTT, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K