3THX
Human MutSbeta complexed with an IDL of 3 bases (Loop3) and ADP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-06-18 |
Detector | RIGAKU SATURN A200 |
Wavelength(s) | 1.54 |
Spacegroup name | P 1 |
Unit cell lengths | 67.120, 91.540, 95.580 |
Unit cell angles | 67.88, 86.51, 72.86 |
Refinement procedure
Resolution | 27.000 - 2.700 |
R-factor | 0.212 |
Rwork | 0.210 |
R-free | 0.27300 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.558 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.000 | 2.750 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.047 | 0.332 |
<I/σ(I)> | 14.8 | 1.44 |
Completeness [%] | 91.8 | 43.3 |
Redundancy | 1.9 | 1.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.2M Ammonium Acetate, 20-25% PEG3350 (w/v) and 0.1M MES pH 6.5-7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |