3THG
Crystal structure of the creosote Rubisco activase C-domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-03-16 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97921 |
Spacegroup name | H 3 2 |
Unit cell lengths | 71.919, 71.919, 151.698 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.130 - 1.880 |
R-factor | 0.21691 |
Rwork | 0.216 |
R-free | 0.23803 |
RMSD bond length | 0.016 |
RMSD bond angle | 1.742 |
Data scaling software | SCALA (3.3.16) |
Phasing software | SHELX |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.135 | 48.135 | 1.980 |
High resolution limit [Å] | 1.880 | 5.950 | 1.880 |
Rmerge | 0.033 | 0.536 | |
Number of reflections | 12496 | ||
<I/σ(I)> | 22.1 | 14.5 | 1.4 |
Completeness [%] | 99.6 | 99.5 | 97.2 |
Redundancy | 9.9 | 10.6 | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 283 | 0.1 M Tris, 0.2 M trimethylamine N-oxide, 20% PEG MME 2000, pH 8.5, vapor diffusion, hanging drop, temperature 283K |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 283 | 0.1 M Tris, 0.2 M trimethylamine N-oxide, 20% PEG MME 2000, pH 8.5, vapor diffusion, hanging drop, temperature 283K |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 283 | 0.1 M Tris, 0.2 M trimethylamine N-oxide, 20% PEG MME 2000, pH 8.5, vapor diffusion, hanging drop, temperature 283K |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 283 | 0.1 M Tris, 0.2 M trimethylamine N-oxide, 20% PEG MME 2000, pH 8.5, vapor diffusion, hanging drop, temperature 283K |