3THF
Crystal structure of the SD2 domain from Drosophila Shroom
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-06-04 |
Detector | ADSC QUANTUM |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 72.797, 85.632, 92.977 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.633 - 2.695 |
R-factor | 0.2317 |
Rwork | 0.228 |
R-free | 0.28380 |
Structure solution method | SAD |
RMSD bond length | 0.100 |
RMSD bond angle | 0.600 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELX |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.750 |
High resolution limit [Å] | 2.695 | 7.320 | 2.695 |
Rmerge | 0.069 | 0.044 | 0.463 |
Number of reflections | 16446 | ||
<I/σ(I)> | 9.7 | ||
Completeness [%] | 99.9 | 99.5 | 99.9 |
Redundancy | 8.5 | 7.7 | 8.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6 | 277 | K/Na tartrate, sodium thiocynate, glycerol, pH 6.0, vapor diffusion, temperature 277K |
1 | VAPOR DIFFUSION | 6 | 277 | K/Na tartrate, sodium thiocynate, glycerol, pH 6.0, vapor diffusion, temperature 277K |