3THC
Crystal structure of human beta-galactosidase in complex with galactose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2009-10-24 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 94.799, 116.104, 140.326 |
Unit cell angles | 90.00, 92.21, 90.00 |
Refinement procedure
Resolution | 28.880 - 1.800 |
R-factor | 0.19217 |
Rwork | 0.190 |
R-free | 0.22833 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3d3a |
RMSD bond length | 0.012 |
RMSD bond angle | 1.430 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 28.880 |
High resolution limit [Å] | 1.800 |
Number of reflections | 250825 |
Completeness [%] | 78.0 |
Redundancy | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 20% (w/v) PEG3350 0.2M ammonium sulfate, 100mM Tris HCl pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |