3TGZ
Crystal Structure Analysis of W35F/H207W Mutant of Human CLIC1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 113 |
Detector technology | CCD |
Collection date | 2009-10-05 |
Detector | Bruker Platinum 135 |
Wavelength(s) | 1.5418 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 157.947, 47.639, 68.097 |
Unit cell angles | 90.00, 106.44, 90.00 |
Refinement procedure
Resolution | 58.290 - 2.300 |
R-factor | 0.2436 |
Rwork | 0.240 |
R-free | 0.30610 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3o3t |
RMSD bond length | 0.014 |
RMSD bond angle | 1.605 |
Data reduction software | SAINT (V7.68A) |
Data scaling software | SAINT (V7.68A) |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 58.290 | 1.952 | |
High resolution limit [Å] | 1.884 | 4.059 | 1.884 |
<I/σ(I)> | 75.242 | ||
Completeness [%] | 98.0 | ||
Redundancy | 8.08 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | hanging drop | 6.5 | 293 | 0.1 M Bis-Tris, 20% (v/v) PEG 3350 monomethyl ether, 0.2 M sodium acetate, 2.5 mM DTT, pH 6.5, hanging drop, temperature 293K |