3TGT
Crystal structure of unliganded HIV-1 clade A/E strain 93TH057 gp120 core
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2010-07-16 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 63.556, 66.944, 88.029 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.571 - 1.900 |
R-factor | 0.191 |
Rwork | 0.189 |
R-free | 0.23070 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.198 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | PHENIX (1.6.1_357) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.930 |
High resolution limit [Å] | 1.900 | 5.160 | 1.900 |
Rmerge | 0.084 | 0.053 | 0.615 |
Number of reflections | 30907 | ||
<I/σ(I)> | 38.5 | 57.9 | 2.1 |
Completeness [%] | 95.2 | 98.1 | 86.5 |
Redundancy | 6.8 | 6.4 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 14% PEG 1500, 12% PEG 400, 0.1M HEPES, VAPOR DIFFUSION, HANGING DROP, temperature 293K, pH 7.5 |