3TGO
Crystal structure of the E. coli BamCD complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-23 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 73.780, 133.380, 144.992 |
| Unit cell angles | 90.00, 100.15, 90.00 |
Refinement procedure
| Resolution | 97.450 - 2.900 |
| R-factor | 0.181 |
| Rwork | 0.177 |
| R-free | 0.24300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2YH6 AND 2YHC |
| RMSD bond length | 0.013 |
| RMSD bond angle | 0.972 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 97.500 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.112 | 0.393 |
| Number of reflections | 30529 | |
| <I/σ(I)> | 14.6 | 5.4 |
| Completeness [%] | 99.6 | 99.8 |
| Redundancy | 7.2 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9.2 | 295 | 0.2 M K2HPO4 and 20% PEG3350, pH 9.2, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
