3TGD
Crystal structure of the human ubiquitin-conjugating enzyme (E2) UbcH5b
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-03-15 |
Detector | RIGAKU SATURN 944+ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 49.342, 50.277, 64.299 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.887 - 1.800 |
R-factor | 0.1812 |
Rwork | 0.179 |
R-free | 0.20280 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2esk |
RMSD bond length | 0.010 |
RMSD bond angle | 1.192 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | PHENIX (1.7_743) |
Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.887 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.043 | 0.080 |
Number of reflections | 15004 | |
<I/σ(I)> | 30.7 | 5.8 |
Completeness [%] | 97.6 | 80.1 |
Redundancy | 6.04 | 2.55 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.8M potassium phosphate, 0.8M sodium phosphate, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |