3TGD

Crystal structure of the human ubiquitin-conjugating enzyme (E2) UbcH5b

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2011-03-15
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	49.342, 50.277, 64.299
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.887 - 1.800
R-factor	0.1812
Rwork	0.179
R-free	0.20280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2esk
RMSD bond length	0.010
RMSD bond angle	1.192
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	PHENIX (1.7_743)
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.887	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.043	0.080
Number of reflections	15004
<I/σ(I)>	30.7	5.8
Completeness [%]	97.6	80.1
Redundancy	6.04	2.55

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.8M potassium phosphate, 0.8M sodium phosphate, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K