3TFY
Naa50p amino-terminal acetyltransferase bound to substrate peptide fragment and CoA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X6A |
Synchrotron site | NSLS |
Beamline | X6A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-06-10 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.1271 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 48.299, 104.103, 68.247 |
Unit cell angles | 90.00, 106.29, 90.00 |
Refinement procedure
Resolution | 33.659 - 2.750 |
R-factor | 0.1935 |
Rwork | 0.190 |
R-free | 0.25370 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2PSW (cofactor and solvent removed) |
RMSD bond length | 0.008 |
RMSD bond angle | 1.596 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.6_289)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.800 |
High resolution limit [Å] | 2.750 | 2.750 |
Number of reflections | 16856 | |
<I/σ(I)> | 10.2 | 2.1 |
Completeness [%] | 98.9 | 93.2 |
Redundancy | 3.6 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 273 | well solution: 16% PEG 8000, 20% glycerol, 40mM potassium phosphate (monobasic), pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 273K |