3TFL
LytR-Cps2a-Psr family protein with bound octaprenyl pyrophosphate lipid
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-02-27 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | .9795 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 73.348, 73.348, 163.869 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.730 - 2.050 |
R-factor | 0.19305 |
Rwork | 0.191 |
R-free | 0.24207 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.285 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.730 | 2.160 |
High resolution limit [Å] | 2.050 | 2.050 |
Number of reflections | 28883 | |
Completeness [%] | 99.9 | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 0.1M HEPES pH 7.5 10% PEG 8K 8% ethylene glycol, VAPOR DIFFUSION, HANGING DROP, temperature 295K |