3TFG

Crystal structure of an H-NOX protein from Nostoc sp. PCC 7120, L66W/L67W double mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-03-29
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.977
Spacegroup name	P 21 3
Unit cell lengths	123.123, 123.123, 123.123
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.531 - 1.900
R-factor	0.1615
Rwork	0.160
R-free	0.18290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3tf8
RMSD bond length	0.018
RMSD bond angle	1.667
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.930
High resolution limit [Å]	1.900	1.900
Number of reflections	47646
Completeness [%]	100.0	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	1.9-2.1 M malonic acid (pH 7.0), VAPOR DIFFUSION, HANGING DROP, temperature 293K