3TF1

Crystal structure of an H-NOX protein from T. tengcongensis under 6 atm of xenon

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-05-30
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.23
Spacegroup name	P 21 21 2
Unit cell lengths	80.128, 130.214, 42.810
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.810 - 2.037
R-factor	0.1818
Rwork	0.180
R-free	0.22100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3tf0
RMSD bond length	0.018
RMSD bond angle	1.517
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.070
High resolution limit [Å]	2.030	2.030
Number of reflections	52373
Completeness [%]	94.7	99.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	20-28% PEG 200, 200-250 mM Sodium Acetate pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K