3TEV
The crystal structure of glycosyl hydrolase from Deinococcus radiodurans R1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-07-23 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97915 |
Spacegroup name | I 4 |
Unit cell lengths | 146.841, 146.841, 108.733 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.300 |
R-factor | 0.183 |
Rwork | 0.181 |
R-free | 0.21780 |
Structure solution method | SAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.362 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.340 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.109 | 0.826 |
Number of reflections | 51087 | |
<I/σ(I)> | 33.8 | 2.76 |
Completeness [%] | 99.9 | 100 |
Redundancy | 7 | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 286 | 24% PEG1500, 20% glycerol, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 286K |