3TEU

Crystal structure of fibcon

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2010-06-17
Detector	ADSC QUANTUM 210
Spacegroup name	P 21 21 21
Unit cell lengths	28.390, 40.070, 61.520
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.576 - 1.002
R-factor	0.1119
Rwork	0.111
R-free	0.13890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	starting model 1FNF
RMSD bond length	0.028
RMSD bond angle	2.531
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.020
High resolution limit [Å]	1.000	1.000
Rmerge	0.047
Number of reflections	37699
Completeness [%]	97.9	90.3
Redundancy	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		4.5	297	0.1 M sodium acetate, 23% PEG8000, 5% PEG 400, 3% dioxane, pH 4.5, VAPOR DIFFUSION, temperature 297K