3TER
Crystal structure of SOAR domain with Inhibition helix from C. elegans
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-04-23 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9791 |
Spacegroup name | P 32 |
Unit cell lengths | 76.988, 76.988, 64.797 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.644 - 2.551 |
R-factor | 0.1985 |
Rwork | 0.191 |
R-free | 0.26210 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.152 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXS |
Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.640 |
High resolution limit [Å] | 2.550 | 2.550 |
Rmerge | 0.047 | |
Number of reflections | 10722 | |
<I/σ(I)> | 19 | |
Completeness [%] | 89.0 | 91.2 |
Redundancy | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.1M HEPES pH 7.0, 15% Tacsimate pH 7.0, 2% PEG 3350 , VAPOR DIFFUSION, SITTING DROP, temperature 293K |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.1M HEPES pH 7.0, 15% Tacsimate pH 7.0, 2% PEG 3350 , VAPOR DIFFUSION, SITTING DROP, temperature 293K |