3TEO
APO Form of carbon disulfide hydrolase (selenomethionine form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 90 |
| Detector technology | PIXEL |
| Collection date | 2008-01-01 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.98089 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 227.180, 165.510, 115.940 |
| Unit cell angles | 90.00, 91.17, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.400 |
| R-factor | 0.22648 |
| Rwork | 0.224 |
| R-free | 0.27091 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.841 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.427 | |
| Number of reflections | 154688 | |
| <I/σ(I)> | 3.2 | |
| Completeness [%] | 98.2 | 99.2 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 301 | 25% PEG 1000, 200 mM LiCl 50 mM phosphate/citrate buffer, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 301K |
