3TEN
Holo form of carbon disulfide hydrolase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 90 |
| Detector technology | PIXEL |
| Collection date | 2008-01-01 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97178 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 204.950, 113.240, 87.140 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.600 |
| R-factor | 0.2675 |
| Rwork | 0.266 |
| R-free | 0.29922 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.110 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | REFMAC |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.700 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.131 | 0.463 |
| Number of reflections | 63162 | |
| Completeness [%] | 99.5 | 99.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 293 | 25% PEG 1000 200 mM LiCl 50 mM phosphate/citrate buffer , pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
