3TEE

Crystal Structure of Salmonella FlgA in open form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2007-06-27
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9830
Spacegroup name	C 2 2 2
Unit cell lengths	107.520, 131.770, 49.360
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.200 - 1.950
R-factor	0.2012
Rwork	0.200
R-free	0.21840
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.060
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	BUSTER (2.8.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.580	2.060
High resolution limit [Å]	1.950	1.950
Number of reflections	25669
<I/σ(I)>	12.3	3.4
Completeness [%]	98.7	98.3
Redundancy	4	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.4	289	18% PEG 2000, 0.8M lithium chloride, 0.05M citric acid, 18% glycerol, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 289K
1	VAPOR DIFFUSION, HANGING DROP	5.4	289	18% PEG 2000, 0.8M lithium chloride, 0.05M citric acid, 18% glycerol, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 289K