3TDW

The GDP complex of the aminoglycoside 2'-phosphotransfere-IIIa F108L mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2007-11-23
Detector	RAYONIX MX-325
Wavelength(s)	0.97946
Spacegroup name	C 1 2 1
Unit cell lengths	83.060, 54.690, 77.810
Unit cell angles	90.00, 108.49, 90.00

Refinement procedure

Resolution	19.210 - 1.700
R-factor	0.1762
Rwork	0.174
R-free	0.22000
RMSD bond length	0.007
RMSD bond angle	1.139
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.6_289))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.000	1.750
High resolution limit [Å]	1.700	1.700
Rmerge	0.066	0.592
Number of reflections	36432
<I/σ(I)>	12.8	2.3
Completeness [%]	99.6	99.8
Redundancy	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	298	0.25 M MgCl2, 0.1 M Tris HCl, 20% (w/v) PEG 4000, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K