3TD7
Crysal structure of the mimivirus sulfhydryl oxidase R596
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-05-29 |
| Detector | Pilatus 6M |
| Wavelength(s) | 0.9766 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 91.306, 91.306, 200.790 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.000 - 2.210 |
| Rwork | 0.227 |
| R-free | 0.25300 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.079 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 2.330 |
| High resolution limit [Å] | 2.210 | 2.210 |
| Number of reflections | 25063 | |
| <I/σ(I)> | 18.9 | 4.4 |
| Completeness [%] | 97.8 | 93.6 |
| Redundancy | 19.3 | 19.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.1 M HEPES, 200 mM magnesium chloride, 200 mM lithium sulfate, 5% (v/v) 2-methyl-2,4-pentanediol (MPD), and 15-20% (w/v) PEG 1500, VAPOR DIFFUSION, HANGING DROP, temperature 293K, pH 7.0 |
