3TCQ
Crystal Structure of matrix protein VP40 from Ebola virus Sudan
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-30 |
| Detector | MARMOSAIC 300 mm CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 63.982, 90.940, 48.684 |
| Unit cell angles | 90.00, 95.86, 90.00 |
Refinement procedure
| Resolution | 27.368 - 1.600 |
| R-factor | 0.19582 |
| Rwork | 0.194 |
| R-free | 0.22052 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4dut |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.590 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.368 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.066 | 0.482 |
| Number of reflections | 36225 | |
| <I/σ(I)> | 10.821 | 2.481 |
| Completeness [%] | 99.4 | 99.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 291 | 11mg/ml EbsuA.17247.a PD00181, 10% PEG4000, 20% glycerol, 0.1M MES pH 6.5. Direct cryoprotection. , VAPOR DIFFUSION, SITTING DROP, temperature 291K |
