3TCK
Crystal structure of Peptidyl-tRNA hydrolase from Mycobacterium tuberculosis - Form 4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2010-07-03 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.54 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 36.300, 38.810, 122.880 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.300 |
Rwork | 0.224 |
R-free | 0.26800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2z2i |
RMSD bond length | 0.008 |
RMSD bond angle | 1.800 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.420 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.158 | |
Number of reflections | 7780 | |
<I/σ(I)> | 11.4 | |
Completeness [%] | 95.4 | |
Redundancy | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Microbatch under oil | 7.5 | 291 | 0.1M HEPES, 15% PEG 8000, 5% isoproponal, pH 7.5, Microbatch under oil, temperature 291K |