3TCJ
CcdB dimer from V. fisheri in complex with one C-terminal domain of F-plasmid CcdA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-12 |
| Detector | MAR CCD 165 mm |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.864, 62.561, 81.951 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.470 - 1.930 |
| R-factor | 0.195 |
| Rwork | 0.192 |
| R-free | 0.25200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3jrz |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.602 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.800 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.075 | 0.452 |
| Number of reflections | 18030 | |
| <I/σ(I)> | 15.6 | 4.07 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.2 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 293 | 8% PEG4000, 0.1M CH3COONa.3H2O, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
