3TCA
Crystal structure of the Ras-associating and pleckstrin-homology domains of RIAM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-26 |
| Detector | ADSC QUANTUM 4r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.860, 82.389, 83.404 |
| Unit cell angles | 90.00, 95.19, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.350 |
| R-factor | 0.236 |
| Rwork | 0.234 |
| R-free | 0.28200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hk0 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.866 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.390 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.059 | 0.275 |
| Number of reflections | 22885 | |
| <I/σ(I)> | 19 | 3.2 |
| Completeness [%] | 91.7 | 98.3 |
| Redundancy | 2.9 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 298 | 12% PEG3350, 100 mM LiAc, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
