3TBI
Crystal structure of T4 gp33 bound to E. coli RNAP beta-flap domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X3A |
| Synchrotron site | NSLS |
| Beamline | X3A |
| Detector technology | CCD |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9785 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 55.218, 112.250, 164.847 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.995 - 3.000 |
| R-factor | 0.25293 |
| Rwork | 0.251 |
| R-free | 0.29485 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.656 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | SnB |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 28.995 |
| High resolution limit [Å] | 3.000 |
| Number of reflections | 10551 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 295 | 0.1 M triethylamine N-oxide, 20% PEG2000 MME, 0.1 M Tris, pH 8.5, VAPOR DIFFUSION, temperature 295K |
