3TBB

Small laccase from Streptomyces viridosporus T7A; alternate crystal form.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2010-12-20
Detector	RAYONIX MX-300
Wavelength(s)	0.97857
Spacegroup name	P 31 2 1
Unit cell lengths	127.000, 127.000, 155.070
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.931 - 2.300
R-factor	0.1632
Rwork	0.161
R-free	0.20550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3tas
RMSD bond length	0.008
RMSD bond angle	1.342
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (dev_764)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.380
High resolution limit [Å]	2.300	4.930	2.300
Rmerge	0.214	0.017	0.017
Number of reflections	64657	6835	6219
<I/σ(I)>	15.12	42.78	2.23
Completeness [%]	99.9	99.3	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	282	Protein concentrationw as 20 mg/ml in Tris-HCl (pH 7.9); the precipitant contained 5% 2-propanol, and 2.5 M dibasic potassium phosphate/monobasic sodium phosphate (pH 5.5)., VAPOR DIFFUSION, HANGING DROP, temperature 282K