3TBA
Structure of Yeast Ribonucleotide Reductase 1 Q288A with dGTP and ADP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-03-28 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.98 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 107.772, 118.086, 67.710 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.130 - 2.800 |
R-factor | 0.20247 |
Rwork | 0.196 |
R-free | 0.26441 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.012 |
RMSD bond angle | 1.569 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.130 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 20226 | |
<I/σ(I)> | 16 | 4 |
Completeness [%] | 96.0 | 94 |
Redundancy | 5.7 | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 0.1M HEPES pH 7.0, 15-25% PEG 3350, 0.2M NaCl, 10 mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 298K |