3TAT
TYROSINE AMINOTRANSFERASE FROM E. COLI
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-01-19 |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | P 32 |
| Unit cell lengths | 126.578, 126.578, 156.243 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 3.500 |
| R-factor | 0.213 |
| Rwork | 0.213 |
| R-free | 0.26000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7aat |
| RMSD bond length | 0.007 |
| RMSD bond angle | 27.100 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.851) |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.800 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.093 | 0.118 |
| Total number of observations | 65956 * | |
| Number of reflections | 13117 | |
| <I/σ(I)> | 14 | 5 |
| Completeness [%] | 88.5 | 1.5 |
| Redundancy | 5 | 1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.8 | pH 6.8 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 2.5 (mg/ml) | |
| 2 | 1 | drop | PEG8000 | 8 (%(w/v)) | |
| 3 | 1 | drop | phenylmethylsulfonyl fluoride | 0.5 (mM) | |
| 4 | 1 | drop | 1,4-dithiothreitol | 0.05 (mM) | |
| 5 | 1 | drop | PLP | 0.05 (mM) | |
| 6 | 1 | drop | phosphate | 12.5 (mM) | |
| 7 | 1 | reservoir | PEG8000 | 10 (%) |
