3TAP
Crystal Structure of Bacillus DNA Polymerase I Large Fragment Bound to Duplex DNA with Cytosine-Adenine Mismatch at (n-3) Position
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-10-23 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97121 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 87.660, 93.270, 105.700 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.719 - 1.655 |
R-factor | 0.1773 |
Rwork | 0.176 |
R-free | 0.20200 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1l5u |
RMSD bond length | 0.009 |
RMSD bond angle | 1.223 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | REFMAC |
Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.760 |
High resolution limit [Å] | 1.650 | 4.950 | 1.650 |
Rmerge | 0.045 | 0.018 | 0.489 |
Number of reflections | 102589 | ||
<I/σ(I)> | 19.92 | 60.76 | 3.43 |
Completeness [%] | 98.1 | 97.9 | 91.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 290 | 45-50% saturated ammonium sulfate, 2.5% MPD, 10 mM magnesium sulfate, 100 mM MES, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 290K |