3TAN
Crystal Structure of Bacillus DNA Polymerase I Large Fragment Bound to Duplex DNA with Cytosine-Adenine Mismatch at (n-1) Position
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 12.3.1 |
Synchrotron site | ALS |
Beamline | 12.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-05-27 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.1110 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 87.430, 93.010, 105.330 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.058 - 1.530 |
R-factor | 0.1704 |
Rwork | 0.170 |
R-free | 0.18450 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1l3t |
RMSD bond length | 0.011 |
RMSD bond angle | 1.358 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | REFMAC |
Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.620 |
High resolution limit [Å] | 1.530 | 4.560 | 1.530 |
Rmerge | 0.033 | 0.015 | 0.480 |
Number of reflections | 127023 | ||
<I/σ(I)> | 26.2 | 77.9 | 4.1 |
Completeness [%] | 97.9 | 85.5 | 99.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 290 | 45-50% saturated ammonium sulfate, 2.5% MPD, 10 mM magnesium sulfate, 100 mM MES, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 290K |