3TAE
5-hydroxycytosine paired with dAMP in RB69 gp43
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-08-15 |
| Detector | MAR scanner 300 mm plate |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 133.274, 123.709, 165.895 |
| Unit cell angles | 90.00, 95.97, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.710 |
| R-factor | 0.22866 |
| Rwork | 0.224 |
| R-free | 0.26792 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.925 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.6.0116) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.700 |
| Number of reflections | 142643 |
| Completeness [%] | 100.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 7% Peg 2000-MME, 100mM NaOAc, 100mM MgSO4, 100mM Hepes, 5% glycerol, 1mM BME, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
