3TAD
Crystal Structure of the Liprin-alpha/Liprin-beta complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-04-01 |
Wavelength(s) | 0.98 |
Spacegroup name | P 61 |
Unit cell lengths | 141.880, 141.880, 181.059 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.980 - 2.900 |
R-factor | 0.2159 |
Rwork | 0.214 |
R-free | 0.25830 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.264 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.900 |
Rmerge | 0.068 |
Number of reflections | 45715 |
Completeness [%] | 99.5 |
Redundancy | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 289 | 3-5% PEG8000, 0.15M NaCl, 0.1M Bis-Tris buffer, pH 6.0, vapor diffusion, hanging drop, temperature 289K |