3TA9
beta-Glucosidase A from the halothermophile H. orenii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-29 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.95375 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 88.504, 99.577, 109.427 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.894 - 3.000 |
| R-factor | 0.1948 |
| Rwork | 0.191 |
| R-free | 0.26620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3FJ0 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.230 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.000 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 19515 | |
| Completeness [%] | 98.4 | 96 |
| Redundancy | 7.9 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.2 M magnesium acetate, 20% PEG8000, 0.1 M sodium cacodylate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
