3TA6
Structure of Mycobacterium tuberculosis triosephosphate isomerase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-20 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.968 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 134.917, 52.589, 77.539 |
| Unit cell angles | 90.00, 104.48, 90.00 |
Refinement procedure
| Resolution | 42.772 - 1.410 |
| R-factor | 0.132 |
| Rwork | 0.131 |
| R-free | 0.15480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.171 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.070 | 1.430 |
| High resolution limit [Å] | 1.410 | 1.410 |
| Number of reflections | 100458 | |
| Completeness [%] | 99.1 | 88.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | 16% PEG3350, 250 mM ammonium citrate, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
