3TA1
A. fulgidus GlnK3, MgADP complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 123.310, 92.000, 88.380 |
| Unit cell angles | 90.00, 133.59, 90.00 |
Refinement procedure
| Resolution | 44.160 - 1.900 |
| R-factor | 0.2135 |
| Rwork | 0.212 |
| R-free | 0.24050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3o8w |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.260 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.9.6) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.020 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.262 | |
| Number of reflections | 56191 | |
| <I/σ(I)> | 10.9 | 2.4 |
| Completeness [%] | 98.7 | 99.6 |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 3.5 | 293 | 17% PEG3350, 0.1 M sodium citrate buffer, pH 3.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
