3T9Z
A. fulgidus GlnK3, ligand-free
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 123.350, 92.760, 88.410 |
| Unit cell angles | 90.00, 133.58, 90.00 |
Refinement procedure
| Resolution | 26.650 - 1.820 |
| R-factor | 0.2087 |
| Rwork | 0.207 |
| R-free | 0.23640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3o8w |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.130 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.9.6) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.400 | 1.920 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Rmerge | 0.191 | |
| Number of reflections | 63525 | |
| <I/σ(I)> | 11 | 3.1 |
| Completeness [%] | 98.2 | 98.7 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 3.5 | 293 | 17% PEG3350, 0.1 M sodium citrate buffer, pH 3.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
