3T9Y
Crystal structure of GNAT family acetyltransferase Staphylococcus aureus subsp. aureus USA300_TCH1516
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-02 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97904 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 69.711, 91.746, 53.866 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.1758 |
| Rwork | 0.173 |
| R-free | 0.22790 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.264 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.075 | 0.480 |
| Number of reflections | 11346 | |
| <I/σ(I)> | 15 | 2 |
| Completeness [%] | 95.0 | 73.7 |
| Redundancy | 4.4 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 297 | 0.2M Lithium sulfate, 0.1M Tris-Cl, 40% PEG400, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
