3T9W

Small laccase from Amycolatopsis sp. ATCC 39116

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2010-03-10
Detector	RAYONIX MX-300
Wavelength(s)	0.97857
Spacegroup name	H 3 2
Unit cell lengths	110.180, 110.180, 178.820
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.657 - 1.500
R-factor	0.1736
Rwork	0.172
R-free	0.20150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3cg8
RMSD bond length	0.008
RMSD bond angle	1.389
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (dev_764)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000	20.000	1.540
High resolution limit [Å]	1.500	6.710	1.500
Rmerge	0.065	0.043	0.810
Number of reflections	66783	782	4905
<I/σ(I)>	18.46	44.58	2.6
Completeness [%]	99.9	94.7	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	Protein concentration was 20 mg/mL buffered with 20 mM Tris (pH 7.9), the precipitant contained 3.0 M NaCl and 100 mM Hepes, VAPOR DIFFUSION, HANGING DROP, temperature 277K