3T9O
Regulatory CZB domain of DgcZ
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-27 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 121.460, 60.260, 38.460 |
| Unit cell angles | 90.00, 99.04, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.200 |
| R-factor | 0.211 |
| Rwork | 0.209 |
| R-free | 0.24600 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.206 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | autoSHARP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.260 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.059 | 0.382 |
| Number of reflections | 27196 | |
| <I/σ(I)> | 10.72 | 2.71 |
| Completeness [%] | 99.3 | 99.7 |
| Redundancy | 2.1 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 200mM ammonium tartrate, 12.5% PEG 3350, pH 8.0, vapor diffusion, hanging drop, temperature 293K |
