3T9N
Crystal structure of a membrane protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-11-06 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 95.029, 138.627, 214.580 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.738 - 3.456 |
R-factor | 0.2544 |
Rwork | 0.253 |
R-free | 0.28810 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2oau |
RMSD bond length | 0.011 |
RMSD bond angle | 1.377 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.570 |
High resolution limit [Å] | 3.445 | 3.445 |
Number of reflections | 36145 | |
Completeness [%] | 95.7 | 93.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 291 | 10%(v/v) 2-Propanol, 0.1M sodium citrate tribasic dehydrate, 22%(w/v) polyethylene glycol 400, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |