3T9N

Crystal structure of a membrane protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2010-11-06
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	95.029, 138.627, 214.580
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.738 - 3.456
R-factor	0.2544
Rwork	0.253
R-free	0.28810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2oau
RMSD bond length	0.011
RMSD bond angle	1.377
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.570
High resolution limit [Å]	3.445	3.445
Number of reflections	36145
Completeness [%]	95.7	93.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	291	10%(v/v) 2-Propanol, 0.1M sodium citrate tribasic dehydrate, 22%(w/v) polyethylene glycol 400, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K