3T8P
Crystal structure of d(CGGGTACCCG)4 as a four-way Holliday junction
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-03-09 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 65.289, 23.597, 37.124 |
| Unit cell angles | 90.00, 111.30, 90.00 |
Refinement procedure
| Resolution | 34.590 - 2.350 |
| R-factor | 0.23759 |
| Rwork | 0.235 |
| R-free | 0.27839 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nt8 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.980 |
| Data reduction software | AUTOMAR |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.590 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.115 | 0.295 |
| Number of reflections | 2236 | |
| <I/σ(I)> | 3.7 | 1.1 |
| Completeness [%] | 96.5 | 96.4 |
| Redundancy | 2.83 | 3.29 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 300 | 1mM DNA, 40mM sodium cacodylate, 85mM Calcium chloride, equilibrated against 30% methyl pentane diol , pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
