3T8K
The crystal structure of a functionally unknown protein Lebu_0176 from Leptotrichia buccalis C-1013-b
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-31 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.193, 101.019, 108.184 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.962 - 1.769 |
| R-factor | 0.1805 |
| Rwork | 0.179 |
| R-free | 0.21520 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.027 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.000 | 1.810 |
| High resolution limit [Å] | 1.769 | 1.780 |
| Rmerge | 0.104 | |
| Number of reflections | 38999 | |
| <I/σ(I)> | 39.6 | 4.3 |
| Completeness [%] | 98.6 | 98.7 |
| Redundancy | 6.7 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.3 | 277 | 0.2M Ammonium Chloride, 20% (w/v) PEG 3350, pH 6.3, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
