3T86
d(GCATGCT) + calcium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-D |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-01-01 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 2 2 2 |
| Unit cell lengths | 22.742, 58.341, 23.669 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.110 - 1.900 |
| R-factor | 0.24135 |
| Rwork | 0.239 |
| R-free | 0.27918 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 2.199 |
| Data reduction software | XFIT |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 29.110 |
| High resolution limit [Å] | 1.900 |
| Number of reflections | 1246 |
| Completeness [%] | 95.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 290 | Drops initially containing 2ul 20 mm sodium cacodylate, 2ul calcium chloride, 2ul potassium chloride, 2 ul 10% PEG, 2ul 1 mM DNA, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
