3T7N
Crystal Structure of Human Glycogenin-1 (GYG1) complexed with manganese and UDP, in a monoclinic closed form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2011-01-19 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 47.020, 80.720, 69.850 |
Unit cell angles | 90.00, 100.92, 90.00 |
Refinement procedure
Resolution | 29.160 - 1.980 |
R-factor | 0.20632 |
Rwork | 0.203 |
R-free | 0.26546 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3q4s |
RMSD bond length | 0.015 |
RMSD bond angle | 1.487 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.430 | 2.090 |
High resolution limit [Å] | 1.980 | 1.980 |
Rmerge | 0.074 | 0.497 |
Number of reflections | 34694 | |
<I/σ(I)> | 10.7 | 2.1 |
Completeness [%] | 97.3 | 95.3 |
Redundancy | 3.3 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293.15 | 20% PEG 3350, 0.2M MgCl2, 0.1M Tris, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K |