3T7L
Crystal structure of the FYVE domain of endofin (ZFYVE16) at 1.1A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-28 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9611 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 42.944, 42.944, 76.879 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.010 - 1.090 |
| R-factor | 0.14244 |
| Rwork | 0.142 |
| R-free | 0.15423 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.676 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.640 | 1.150 |
| High resolution limit [Å] | 1.090 | 1.090 |
| Rmerge | 0.082 | 0.748 |
| Number of reflections | 30734 | |
| <I/σ(I)> | 10.8 | 2 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 5.2 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277.15 | 20% PEG 3350, 0.2 M NaNO3, 10% Ethylene glycol, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277.15K |
