3T7K

Complex structure of Rtt107p and phosphorylated histone H2A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2011-04-14
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9790
Spacegroup name	P 1
Unit cell lengths	34.517, 59.255, 85.890
Unit cell angles	78.36, 89.68, 73.17

Refinement procedure

Resolution	30.548 - 2.028
R-factor	0.2201
Rwork	0.218
R-free	0.25760
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.133
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER (2.2.4)
Refinement software	PHENIX (1.6.1_357)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.070
High resolution limit [Å]	2.028	5.510	2.028
Rmerge	0.076	0.052	0.560
Number of reflections	39702
<I/σ(I)>	8.8
Completeness [%]	96.5	94.5	95.6
Redundancy	2.9	2.9	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.8	283	14%(w/v) PEG 1500, 0.2M NaCl, 0.1M MES, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 283K