3T7G
Atg8 transfer from Atg7 to Atg3: a distinctive E1-E2 architecture and mechanism in the autophagy pathway
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Detector technology | CCD |
| Collection date | 2009-12-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 152.135, 99.429, 71.502 |
| Unit cell angles | 90.00, 113.13, 90.00 |
Refinement procedure
| Resolution | 33.212 - 2.080 |
| R-factor | 0.1829 |
| Rwork | 0.182 |
| R-free | 0.20560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3t7f |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.033 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.080 |
| Number of reflections | 58224 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 295 | 1.8M ammonium sulfate, 0.1M Bis-Tris pH 5.5, 5mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
