3T7E

Atg8 transfer from Atg7 to Atg3: a distinctive E1-E2 architecture and mechanism in the autophagy pathway

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Detector technology	CCD
Collection date	2011-06-23
Detector	ADSC QUANTUM 315
Wavelength(s)	1.28255
Spacegroup name	P 43 21 2
Unit cell lengths	66.556, 66.556, 222.268
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.512 - 2.250
R-factor	0.1965
Rwork	0.193
R-free	0.23330
Structure solution method	MR-SAD
RMSD bond length	0.008
RMSD bond angle	1.205
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.250
Number of reflections	24766

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	12% PEG 20K, 0.1M Tris pH 8.5, 10mM TCEP, VAPOR DIFFUSION, HANGING DROP, temperature 277K