3T72
PhoB(E)-Sigma70(4)-(RNAP-Betha-flap-tip-helix)-DNA Transcription Activation Sub-Complex
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2006-06-27 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.2542,1.2554,1.2498 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 277.300, 161.400, 260.100 |
Unit cell angles | 90.00, 91.40, 90.00 |
Refinement procedure
Resolution | 50.000 - 4.330 |
Structure solution method | MAD |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHELXS |
Refinement software | REFMAC |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 4.330 |
Number of reflections | 73615 |
Completeness [%] | 100.0 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 8-10% PEG 4000, 100 mM KCl, 10 mM magnesium chloride, 50 mM MES, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |